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In July-August 2018, we collected 88 filtered pore water samples at 20 cm depth and analyzed them for anions, cations, dissolved organic carbon, dissolved organic matter (DOM) quality, and standard water quality parameters. Vertical hydraulic head gradients were measured to assess the potential for upward or downward water flow and sediment grab samples were taken for grain size analyses to constrain the relationship between streambed morphology and water flow. Lastly, DNA was extracted from pore water filters for a 16S rRNA gene sequencing analysis. This allowed for a high-resolution characterization of streambed hydrology and biogeochemistry during base flow. Data.csv includes coordinates, time of sampling, grain size analysis D10 values, vertical hydraulic head gradients, microbiological diversity metrics, and multiple chemical analyses. ReadMe.txt contains all information regarding sampling procedure, analysis instrumentation, and reporting limits. Microbiological sequences analyzed here have been deposited in the NCBI Sequence Read Archive and may be accessed using accession number PRJNA527829.
Knowledge graph centered on Distributed hydrological, chemical, and microbiolo with 10 nodes and 39 connections. Top connected: Crested Butte, East River, drive-point piezometer groundwater sampling, DNA metabarcoding, Loss-on-ignition method.
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