Triangulating from the cairns…
Knowledge graph centered on PCR screening with 59 nodes and 115 connections. Top connected: Scarlet Gilia, Slender-tube Skyrocket, Beetles, Pekania pennanti, Lepidoptera.
PCR amplification using 2 μL DNA template, 1 unit Taq polymerase, 2 pmol primers, 5 nmol dNTPs in 25 μL reaction volume with hot start at 94°C for 2 min, 30 cycles with 55°C anneal and 72°C elongation
Synthesized from method descriptions across 1 paper using this protocol.