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Knowledge graph centered on PCR screening with 60 nodes and 117 connections. Top connected: Rocky Mountain Biological Laboratory, Beetles, Ipomopsis aggregata, Lepidoptera, climatological variability.
PCR amplification using 2 μL DNA template, 1 unit Taq polymerase, 2 pmol primers, 5 nmol dNTPs in 25 μL reaction volume with hot start at 94°C for 2 min, 30 cycles with 55°C anneal and 72°C elongation
Synthesized from method descriptions across 1 paper using this protocol.