DNA extraction with DNeasy PowerSoil Kit

Local Knowledge Graph (Top 68 entities)

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Knowledge graph centered on DNA extraction with DNeasy PowerSoil Kit with 68 nodes and 148 connections. Top connected: Salix lucida subsp. lasiandra, Bacteria, Lupinus prunophilus, Lupinus caespitosus, Organic matter decomposition.

Method synopsis

DNA extraction from 5g soil samples in two batches with modifications including vortexing in sodium dodecyl sulfate reagent and incubation at 60°C

Synthesized from method descriptions across 2 papers using this protocol.

Papers Using This Protocol (2)

article

Microbial communities across a hillslope-riparian transect shaped by proximity to the stream, groundwater table, and weathered bedrock

2019Ecology and Evolution

thesis

The structure and function of subalpine ecosystems in the face of climate change

2012

Species Studied With This Protocol

Places Where Used

Crested Butte

town1 paper

Upper Colorado River Basin

watershed1 paper

Colorado

state1 paper

Rocky Mountain Biological Laboratory

study site1 paper

East River Watershed

watershed1 paper

East River

watershed1 paper

PLM0

study site1 paper

PLM1

study site1 paper

PLM2

study site1 paper

PLM3

study site1 paper

DNA extraction with DNeasy PowerSoil Kit

Local Knowledge Graph (Top 68 entities)

Method synopsis

Papers Using This Protocol (2)

Microbial communities across a hillslope-riparian transect shaped by proximity to the stream, groundwater table, and weathered bedrock

The structure and function of subalpine ecosystems in the face of climate change

Species Studied With This Protocol

Lupinus caespitosus

Salix lucida subsp. lasiandra

Bromus

Bacteria

Lupinus prunophilus

Senecio

Heliomeris

Lathyrus lanszwertii var. leucanthus

Castilleja miniata

Taraxacum officinale

Places Where Used

Crested Butte

Upper Colorado River Basin

Colorado

Rocky Mountain Biological Laboratory

East River Watershed

East River

PLM0

PLM1

PLM2

PLM3