DNA extraction with DNeasy PowerSoil Kit

Local Knowledge Graph (Top 68 entities)

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Knowledge graph centered on DNA extraction with DNeasy PowerSoil Kit with 68 nodes and 146 connections. Top connected: Rocky Mountain Biological Laboratory, Bacteria, Interspecies competition, Organic matter decomposition, Temperature.

Method synopsis

DNA extraction from 5g soil samples in two batches with modifications including vortexing in sodium dodecyl sulfate reagent and incubation at 60°C

Synthesized from method descriptions across 2 papers using this protocol.

Papers Using This Protocol (2)

article

Microbial communities across a hillslope-riparian transect shaped by proximity to the stream, groundwater table, and weathered bedrock

2019Ecology and Evolution

thesis

The structure and function of subalpine ecosystems in the face of climate change

2012

Species Studied With This Protocol

Places Where Used

Rocky Mountain Biological Laboratory

study site2 papers

Colorado

state2 papers

Crested Butte

town1 paper

North America

region1 paper

Sierra Nevada

region1 paper

Rocky Mountains

region1 paper

Gunnison County

county1 paper

Upper Colorado River Basin

watershed1 paper

Colorado Rocky Mountains

region1 paper

Gothic

town1 paper

DNA extraction with DNeasy PowerSoil Kit

Local Knowledge Graph (Top 68 entities)

Method synopsis

Papers Using This Protocol (2)

Microbial communities across a hillslope-riparian transect shaped by proximity to the stream, groundwater table, and weathered bedrock

The structure and function of subalpine ecosystems in the face of climate change

Species Studied With This Protocol

Bromus

Pseudomonas fluorescens

Gammaproteobacteria

Deltaproteobacteria

Artemisia tridentata ssp. vaseyana

Artemisia tridentata

Delphinium barbeyi

Betaproteobacteria

Androsace septentrionalis

Megascapheus bottae

Places Where Used

Rocky Mountain Biological Laboratory

Colorado

Crested Butte

North America

Sierra Nevada

Rocky Mountains

Gunnison County

Upper Colorado River Basin

Colorado Rocky Mountains

Gothic