Checking billy barr’s snow log…
Knowledge graph centered on bacterial isolation and preparation with 63 nodes and 123 connections. Top connected: Rocky Mountain Biological Laboratory, Climate Change Impacts, Interspecies competition, Organic matter decomposition, Temperature.
Grew bacterial strains in isolation at constant temperature 25°C in 5 ml of R2A medium, pelleted and re-suspended in sterile water to OD600 of 0.01
Synthesized from method descriptions across 1 paper using this protocol.