radioimmunoassay (Sciuridae)

Knowledge graph centered on radioimmunoassay (Sciuridae) with 71 nodes and 165 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Marmota, Yellow-bellied Marmot, Crested Butte.

Method synopsis

Progesterone concentrations measured using radioimmunoassay with tritiated progesterone, ether extraction, and antibody binding assay. Standard curves and duplicate samples used for quantification.

Synthesized from method descriptions across 19 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Nonlinear phenomena in marmot alarm calls: a mechanism encoding fear? (2025), Philosophical Transactions of the Royal Society B. The protocol was originally introduced by Fecal glucocorticoid metabolites in wild yellow-bellied marmots: experimental validation, individual differences and ecological correlates (2012), General and Comparative Endocrinology. Implementations in other papers (listed below) may differ.

1. Collect physiological samples
   Faecal samples were collected when marmots defecated during trapping. Samples were analyzed for glucocorticoid metabolites using validated assay and parasite infection status (Eimeria).
   Quantities: Faecal samples from trapped individualsDuration: During trapping sessionsConditions: When marmots defecated during handling
   Equipment: sample collection containers, validated glucocorticoid assay

Typical Equipment

• tritiated progesterone (#337, G.D. Niswender, Colorado State University, Fort Collins, CO)
• antibody at working dilution of 1:6000
• dextran-coated charcoal
• centrifuge

Output Measurements

• progesterone concentration (ng/ml)

Papers Using This Protocol (19)

Species Studied With This Protocol

Places Where Used