Knowledge graph centered on hand-pollination crossing (Orobanchaceae) with 54 nodes and 165 connections. Top connected: Hummingbird, Ipomopsis, Delphinium, I. aggregata, Ipomopsis aggregata.
Controlled laboratory assay using prepared stigmas on growth medium to test pollen germination and tube growth across species boundaries. Stigmas are prepared from unopened buds, treated with pure pollen loads, and incubated under controlled conditions to observe tube growth.
Synthesized from method descriptions across 2 papers using this protocol.
Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Phylogeny does not predict the outcome of heterospecific pollen–pistil interactions in a species-rich alpine plant community (2025), American Journal of Botany. Implementations in other papers (listed below) may differ.