Ovary response measurement for fertilization assessment

Knowledge graph centered on Ovary response measurement for fertilization assessment with 69 nodes and 150 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Buff-tailed Bumble Bee, Golden-belted Bumble Bee, Common Eastern Bumble Bee.

Method synopsis

Post-pollination measurement of ovary size changes as a proxy for detecting fertilization events and ovule development. Uses standardized photography and image analysis to quantify ovary dimensions.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Phylogeny does not predict the outcome of heterospecific pollen–pistil interactions in a species-rich alpine plant community (2025), American Journal of Botany. Implementations in other papers (listed below) may differ.

1. Measure ovary response to pollination treatments
   Measured the size of ovaries as a proxy for detecting ovule fertilization in a pollination treatment. Ovaries were photographed on a dissecting microscope with a Leica camera at a maximum of 40× magnification. All images included a scale bar for accurately measuring ovary length, width, and area using ImageJ2. Measured the length of the ovary as the longest single dimension of the ovary and the width at the widest section perpendicular to the measured length.
   Quantities: 40× maximum magnification for ovary imaging, length and width measurements in perpendicular dimensionsDuration: Individual photography per ovaryConditions: Dissecting microscope setup with scale bar inclusion
   Equipment: dissecting microscope, Leica camera, ImageJ2 software

Typical Equipment

• dissecting microscope
• Leica camera
• ImageJ2 software

Output Measurements

• ovary length
• ovary width
• ovary area

Papers Using This Protocol (2)

Species Studied With This Protocol

Places Where Used