McGonigle method (Poaceae)

Knowledge graph centered on McGonigle method (Poaceae) with 71 nodes and 171 connections. Top connected: Ligusticum porteri, phenological mismatch, Potentilla, Rushes, Bacteria.

Method synopsis

Microscopic assessment of arbuscular mycorrhizal fungal colonization and other fungal colonization of plant roots using crosshair intercept method and grid line-intercept method for soil fungi.

Synthesized from method descriptions across 23 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Experimental warming decouples plant-fungal symbiont interactions and leads to a more conservative ecosystem (2026), PNAS. Implementations in other papers (listed below) may differ.

1. Measure root fungal colonization
   All plants in plots were assessed for percent colonization of AM fungal hyphae (aseptate hyphae) and other fungi (septate hyphae), as well as AM fungal organelles (arbuscules and vesicles) via crosshair intercept method. Fungal hyphal length of AM fungi and other fungi were counted in 5 g of soil using the grid line-intercept method with 50 fields of view per sample.
   Quantities: 5 g soil per sample, 50 fields of view per sampleDuration: Laboratory analysis following field collectionConditions: Laboratory conditions
   Equipment: microscope, grid line-intercept setup

Typical Equipment

• microscope
• grid line-intercept setup

Output Measurements

• AM fungal colonization
• septate fungal colonization
• fungal hyphal length

Papers Using This Protocol (23)

Species Studied With This Protocol