Paired invasion plot soil sampling (Asteraceae)

Knowledge graph centered on Paired invasion plot soil sampling (Asteraceae) with 39 nodes and 125 connections. Top connected: Spruce, Asteraceae, Bacteria, native species, Linaria vulgaris.

Method synopsis

Systematic collection of soil samples from paired invaded and uninvaded plots to compare soil properties and microbial communities affected by plant invasion history.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Legacy effects and arbuscular mycorrhizal fungi of Linaria vulgaris invasion in Colorado and Illinois, USA (2025), Invasive Plant Science and Management. Implementations in other papers (listed below) may differ.

1. Identify and establish study plots
   Identified populations of L. vulgaris from research records and iNaturalist reports. In summer 2020, collected soil samples from 10 paired plots of L. vulgaris-invaded and adjacent uninvaded plots in each site. Paired plots were at least 75 m apart and were demarcated using N-S and E-W tape measurements to calculate plot area.
   Quantities: 10 paired plots per site (20 total plots), plots at least 75 m apart, plots greater than 100 m² were subsampled from centermost 5-m² areaDuration: Summer 2020 (July to August)Conditions: Field sampling conducted during summer growing season
   Equipment: N-S and E-W tape measures
2. Collect soil and rhizome samples
   Collected 160 soil cores (152.4-mm depth, 101.6-mm width) from each site (IL and CO), with 8 samples from each of 10 invaded plots and 10 adjacent uninvaded plots. In invaded plots, soil was collected from directly beneath the nearest L. vulgaris individuals, while in uninvaded plots, soil was collected beneath the nearest plants. Also collected 32 rhizomes (each 2.4-cm long) of L. vulgaris from each invaded plot.
   Quantities: 160 soil cores per site (320 total), 152.4-mm depth, 101.6-mm width, 32 rhizomes per site (2.4-cm long each), total of 640 rhizomesDuration: Single sampling event in summer 2020Conditions: Bulk soil and rhizomes collected from subalpine meadows (~2895 m asl) in CO and semi-urban natural areas (~178 m asl) in IL
   Equipment: soil corer (152.4-mm depth, 101.6-mm width), measuring tools for rhizomes

Typical Equipment

• N-S and E-W tape measures
• soil corer

Output Measurements

• soil chemistry
• soil texture
• AMF community composition

Papers Using This Protocol (1)

Species Studied With This Protocol