soil incubation (Asteraceae)

Knowledge graph centered on soil incubation (Asteraceae) with 72 nodes and 185 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Engelmann Spruce, Pekania pennanti, Asteraceae.

Method synopsis

Factorial greenhouse experiment testing effects of soil inoculum source and sterilization on alpine grass growth, traits, and fungal colonization. Plants grown in standardized sand-inoculum mixtures with controlled watering and fertilization.

Synthesized from method descriptions across 8 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Legacy effects and arbuscular mycorrhizal fungi of Linaria vulgaris invasion in Colorado and Illinois, USA (2025), Invasive Plant Science and Management. The protocol was originally introduced by Won't you be my neighbor: neighborhood effect influences mycorrhizal and endophyte colonization (2013). Implementations in other papers (listed below) may differ.

1. Growth chamber experimental setup
   Complete factorial design (2×2×2) measuring growth and N uptake in Linaria and two native plant species. Pots contained either L. vulgaris or Rudbeckia hirta and Deschampsia cognata grown together. Soil treatments: CO or IL; invasion history: uninvaded or L. vulgaris-invaded soil; and N fertilization: ambient or N-fertilized (N kg ha⁻¹). Results in eight treatments with 10 replicate pots per treatment and plant species (n = 160).
   Quantities: 160 pots total, 8 treatments, 10 replicates per treatment, 2 plant species combinationsDuration: 120 days growth periodConditions: 12/12-h photoperiod (day/night), 25/15°C (day/night), 75% relative humidity for 4 mo
   Equipment: growth chambers, 10-L pots

Typical Equipment

• ~150 mL plastic root trainer pots
• QUIKRETE Play Sand
• automatic overhead spraying system
• FloraGro fertilizer

Output Measurements

• plant biomass
• plant height
• specific leaf area
• root:shoot ratio

Papers Using This Protocol (8)

Species Studied With This Protocol

Places Where Used