15N tracer uptake (Asteraceae)

Knowledge graph centered on 15N tracer uptake (Asteraceae) with 39 nodes and 125 connections. Top connected: Spruce, Asteraceae, Bacteria, native species, Linaria vulgaris.

Method synopsis

Stable isotope tracer experiment using 15N-labeled fertilizer to quantify nitrogen uptake rates and allocation in plants growing under different soil treatments.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Legacy effects and arbuscular mycorrhizal fungi of Linaria vulgaris invasion in Colorado and Illinois, USA (2025), Invasive Plant Science and Management. Implementations in other papers (listed below) may differ.

1. Stable isotope tracer experiment
   At the experimental midpoint, 5 ml of 14.37 mM ¹⁵NH₄-¹⁵NO₃ solution (>98.9 at %¹⁵N; Cambridge Stable Isotopes, Tewksbury, MA, USA) was injected into the root zone of each pot using 100-mm 11-gauge biopsy needles, leading to a total of 2.012 mg N added per pot. After 24 h, plants were destructively harvested by cutting at the soil surface into root and shoot fractions, dried in biomass ovens (72 h, 80°C), and weighed.
   Quantities: 5 ml solution per pot, 14.37 mM concentration, 2.012 mg N total added per pot, 24 hour uptake periodDuration: 24 hours between injection and harvest, 72 hours drying timeConditions: 80°C drying temperature, injection at experimental midpoint
   Equipment: 100-mm 11-gauge biopsy needles (Fisher Scientific, Waltham, MA, USA), biomass ovens, Cambridge Stable Isotopes ¹⁵NH₄-¹⁵NO₃, Costech 4010 ECS, IRMS Delta V Plus spectrometer

Typical Equipment

• 100-mm 11-gauge biopsy needles
• Cambridge Stable Isotopes ¹⁵NH₄-¹⁵NO₃
• Costech 4010 ECS
• IRMS Delta V Plus spectrometer

Output Measurements

• nitrogen uptake rate
• net uptake rate
• plant tissue 15N enrichment

Papers Using This Protocol (1)

Species Studied With This Protocol