Dual-domain column redox microsite simulation

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Method synopsis

Laboratory column experiments with embedded artificial reducing microsites in natural sediments under controlled flow conditions to test anoxic microsite detection methods.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure as described in the canonical source

Steps below were extracted from the paper that introduces this protocol — X-ray chemical imaging for assessing redox microsites within soils and sediments (2024), Frontiers in Environmental Chemistry. Implementations in other papers (listed below) may differ.

1. Collect natural sediments for column experiments
   Natural sediments were collected 2 weeks prior from the Wind River-Little Wind River floodplain outside of Riverton, WY (42°59'19.1"N, 108°23'58.6"W). Columns (30 cm long, 7 cm diameter) were filled with natural sediments.
   Quantities: Columns: 30 cm length, 7 cm diameterDuration: Collection 2 weeks prior to experimentConditions: Field collection from floodplain
   Equipment: columns
2. Embed artificial microsites in column
   One fine-grained, organic-rich, reducing lens (~3 cm diameter, 17-20 g dry weight made from sulfidic sediments collected underneath an oxbow lake on the floodplain) was embedded into aquifer sand (1.3-1.4 kg dry sand in the column).
   Quantities: Lens: ~3 cm diameter, 17-20 g dry weight; Sand: 1.3-1.4 kg dryDuration: Single setupConditions: Laboratory setup
   Equipment: sulfidic sediments, aquifer sand
3. Run column flow experiment
   Columns were fed artificial groundwater (in upflow mode using a peristaltic pump) at a rate of 90 mL/day, equivalent to ~1 pore volume exchange per 48 h. The column experiment was run for a total of 70 days.
   Quantities: Flow rate: 90 mL/day, ~1 pore volume per 48 h

Typical Equipment

• columns
• peristaltic pump
• artificial groundwater

Output Measurements

• spatial distribution of artificial microsites
• redox gradients

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used