Anoxic sample preparation and sectioning

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Method synopsis

Specialized sample sectioning and epoxy stabilization under anoxic conditions to preserve redox-sensitive microsites for X-ray analysis.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure as described in the canonical source

Steps below were extracted from the paper that introduces this protocol — X-ray chemical imaging for assessing redox microsites within soils and sediments (2024), Frontiers in Environmental Chemistry. Implementations in other papers (listed below) may differ.

1. Section samples for X-ray analysis
   The dual domain column and natural soil cores were cut in half (lengthwise) in a two-step process: (i) the PVC 'shell' was cut with a rotary cutter and (ii) the frozen column/soil cores were cut with a sharp knife. For each sample, one half of the cut core was selected for X-ray imaging and a ~2-mm section of the surface was removed delicately using a knife to further remove any smearing or contamination from the cutting process.
   Quantities: ~2-mm section removed from surfaceDuration: Single preparation per sampleConditions: Samples kept frozen until sectioning
   Equipment: rotary cutter, sharp knife
2. Stabilize samples with epoxy
   Soil core halves were left to thaw and dry partially, inside the anoxic chamber at ambient temperature under suction (vacuum) for 24-48 hours, prior to being fixed with epoxy (EPO-TEK 301-2FL). Samples were covered in epoxy to stabilize the sand. Before the epoxy fully hardened (~12 h), ~2-10 mm diameter and ~1-2 mm deep holes were carved along the surface of each synthetic core, using a simple needle.
   Quantities: Holes: ~2-10 mm diameter, ~1-2 mm deepDuration: 24-48 hours drying, ~12 hours epoxy hardeningConditions: Anoxic chamber, ambient temperature, vacuum
   Equipment: anoxic chamber, vacuum, EPO-TEK 301-2FL epoxy, needle

Typical Equipment

• anoxic chamber
• EPO-TEK 301-2FL epoxy
• rotary cutter
• sharp knife

Output Measurements

• preserved soil sections
• stable sample surfaces

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used