CRISPR-Cas9 gene editing (Pieridae)

Knowledge graph centered on CRISPR-Cas9 gene editing (Pieridae) with 70 nodes and 157 connections. Top connected: Ligusticum porteri, Atriplex canescens, Lepidoptera, Populus, Boechera stricta.

Method synopsis

DNA extraction, tagmentation-based library preparation, and Illumina sequencing to generate whole-genome sequence data from butterfly specimens. Modified DNeasy protocol followed by Tn5 tagmentation and PCR amplification.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Genetic basis of an adaptive polymorphism controlling butterfly silver iridescence (2025), Current Biology. Implementations in other papers (listed below) may differ.

1. Genome sequencing library preparation
   Generated sequencing libraries using PE 150 protocol and sequenced on Illumina NovaSeq6000 S4 Flow cell at Institute for Genome Sciences, Baltimore generating ~11% mean coverage
   Quantities: 70 samples, ~11% mean coverageDuration: Not specifiedConditions: Standard Illumina sequencing conditions
   Equipment: Illumina NovaSeq6000, bwa-mem, picard, GATK HaplotypeCaller

Typical Equipment

• DNeasy Blood & Tissue Kit
• NanoDrop 2000/2000c
• Tn5 transposase
• Start Taq DNA polymerase
• thermal cycler
• AMPureXP beads
• Illumina NovaSeq S4

Output Measurements

• whole-genome sequence reads
• SNP genotypes

Papers Using This Protocol (4)

Species Studied With This Protocol