Knowledge graph centered on CRISPR-Cas9 gene editing (Pieridae) with 70 nodes and 157 connections. Top connected: Ligusticum porteri, Atriplex canescens, Lepidoptera, Populus, Boechera stricta.
DNA extraction, tagmentation-based library preparation, and Illumina sequencing to generate whole-genome sequence data from butterfly specimens. Modified DNeasy protocol followed by Tn5 tagmentation and PCR amplification.
Synthesized from method descriptions across 4 papers using this protocol.
Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Genetic basis of an adaptive polymorphism controlling butterfly silver iridescence (2025), Current Biology. Implementations in other papers (listed below) may differ.