Biolog EcoPlate assay

Knowledge graph centered on Biolog EcoPlate assay with 24 nodes and 95 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Boechera stricta, Wyethia amplexicaulis, soil microbial community structure.

Method synopsis

Community-level physiological profiling using 96-well plates with 31 different carbon sources to assess functional diversity of soil microbial communities.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Context dependence of warming induced shifts in montane soil microbial functions (2024), Functional Ecology. Implementations in other papers (listed below) may differ.

1. Measure community-level physiological profiles
   4 g of soil was suspended with 50 mL potassium phosphate buffer and diluted, then added to 96-well EcoPlate plates. Plates were incubated and analyzed using Synergy HT BioTek plate reader to measure functional diversity via richness of positive tests after background correction.
   Quantities: 4 g soil, 50 mL buffer, 96-well platesDuration: Incubation period for plate developmentConditions: Functional diversity calculated from 31 carbon sources
   Equipment: Biolog EcoPlate assay, Synergy HT BioTek plate reader

Typical Equipment

• Biolog EcoPlate assay
• Synergy HT BioTek plate reader

Output Measurements

• functional diversity
• metabolic activity

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used