Chloroform fumigation extraction

Knowledge graph centered on Chloroform fumigation extraction with 71 nodes and 151 connections. Top connected: Spruce, Atriplex canescens, Potentilla, Rushes, Bacteria.

Method synopsis

Standard chloroform fumigation direct extraction technique for measuring soil microbial biomass, combined with soil chemistry analysis and enzyme activity assays.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Experimental warming decouples plant-fungal symbiont interactions and leads to a more conservative ecosystem (2026), PNAS. Implementations in other papers (listed below) may differ.

1. Measure soil properties and microbial biomass
   Soil resources including plant-available soil nutrients (phosphate, nitrate, and ammonium), soil organic matter, soil pH, and gravimetric soil moisture of each soil sample were quantified from each of the 10-cm increment samples collected above. Soil microbial biomass was extracted using chloroform fumigation direct extraction technique. Microbial enzymes involved in carbon, nitrogen, and phosphorus cycling were assayed.
   Quantities: 10 cm increment samples from 1 m depthDuration: Laboratory analysis following field collectionConditions: Laboratory conditions
   Equipment: chloroform fumigation extraction setup, soil chemistry analysis equipment

Typical Equipment

• chloroform fumigation extraction setup
• soil chemistry analysis equipment

Output Measurements

• soil microbial biomass
• soil nutrients
• soil pH
• soil moisture
• enzyme activity

Papers Using This Protocol (4)

Species Studied With This Protocol