Flow cytometry nuclear DNA content analysis (Salicaceae)

Knowledge graph centered on Flow cytometry nuclear DNA content analysis (Salicaceae) with 70 nodes and 175 connections. Top connected: Spruce, Atriplex canescens, phenological mismatch, Asteraceae, Populus.

Method synopsis

Cytotype inference using twelve multinational microsatellites with 97% correct classification for cytotype. Individual plants defined as diploid or triploid based on maximum number of alleles observed across markers.

Synthesized from method descriptions across 5 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Relaxation of the leaf economics spectrum within and across quaking aspen Populus tremuloides genotypes (2025), Oikos. The protocol was originally introduced by Cytotype and genotype predict mortality and recruitment in Colorado quaking aspen (<i> Populus tremuloides </i>) (2021), Ecological Applications. Implementations in other papers (listed below) may differ.

1. Determine cytotype and genotype
   Cytotype (diploid or triploid) and genotype were determined for 2-3 leaves for each focal tree based on amplification of twelve multinational microsatellites. DNA extraction and microsatellite amplification followed established protocols. Individual plants were defined as putative diploid when maximum of only two alleles were observed at each marker, and as triploid if three alleles were observed at at least one of ten markers.
   Quantities: 2-3 leaves per tree, 12 microsatellite markers, 172 trees totalDuration: Not specifiedConditions: Laboratory conditions
   Equipment: microsatellite markers, DNA extraction equipment, PCR amplification equipment

Typical Equipment

• microsatellite markers
• DNA extraction equipment
• PCR amplification equipment

Output Measurements

• cytotype classification
• genotype identification

Papers Using This Protocol (6)

Species Studied With This Protocol