pavo visual modeling framework

Knowledge graph centered on pavo visual modeling framework with 56 nodes and 164 connections. Top connected: Hummingbird, Ligusticum porteri, Atriplex canescens, Lepidoptera, Bacteria.

Method synopsis

Photography of butterfly landing sites using polarizing lens to measure landscape contrast between landing spots and surrounding areas. Photos analyzed using ImageJ to calculate gray scale contrast values.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Two sides of the same wing: ventral scales enhance dorsal wing color in the butterfly Speyeria mormonia (2023), Journal of Experimental Biology. The protocol was originally introduced by Does an achromatic border alter color discrimination of male <i> Speyeria mormonia </i> butterflies during mate searching? (2022). Implementations in other papers (listed below) may differ.

1. Wing preparation and clamping
   Removed left forewing and left hindwing from each individual. Clamped wings between two metal slides with circular cutout in middle. Held wings stable while preventing unwanted reflections from materials in front of or behind the wings.
   Quantities: Left forewing and hindwing from each of 20 individualsDuration: Not specifiedConditions: Wing surfaces approximately normal to viewing axis of microscope objective
   Equipment: two metal slides with circular cutouts
2. Initial reflectance measurement
   Measured spectral reflectance of dorsal patches on wings using modified Olympus CX-31 microscope with 10X PlanC N UIS2 objective, 20W tungsten halogen lamp, and Flame-S-VIS-NIR-ES spectrometer. Took reliable UV reflectance measurements but limited analysis to 400-700 nm range.
   Quantities: One marginal orange patch and one submarginal orange patch for one forewing and one hindwing from each of 20 individualsDuration: Not specifiedConditions: Standardized using SpectraSuite software with WS-1-SL reference standard
   Equipment: modified Olympus CX-31 microscope, 10X PlanC N UIS2 objective, 20W tungsten halogen lamp, Flame-S-VIS-NIR-ES spectrometer, Ocean Optics, SpectraSuite software, WS-1-SL reference standard

Typical Equipment

• camera with polarizing lens
• colored flags
• ImageJ software

Output Measurements

• landscape contrast values
• gray scale differences
• sun angle measurements

Papers Using This Protocol (5)

Species Studied With This Protocol