Rhizon porewater sampling for redox-sensitive colloids

Knowledge graph centered on Rhizon porewater sampling for redox-sensitive colloids with 16 nodes and 33 connections. Top connected: Crested Butte, Pekania pennanti, Bacteria, Structure and composition of natural ferrihydrite , EXAFS spectroscopy.

Method synopsis

Anoxic porewater collection using Rhizon samplers at 130-180 cm depth with immediate filtration through multiple membrane sizes under N2-purged conditions to preserve redox state of Fe-bearing colloids.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Structure and composition of natural ferrihydrite nano-colloids in anoxic groundwater (2023), Water Research. Implementations in other papers (listed below) may differ.

1. Install Rhizon soil moisture samplers
   Rhizon soil moisture samplers (0.6 μm pore size, 19.60.21F, Rhizosphere Research Products) were installed at depths of 130 to 180 cm and modified to connect to surface sampling ports with aluminum fittings and phthalate tubing to minimize intrusion along sampling lines
   Quantities: Multiple samplers at 130-180 cm depthDuration: Not specifiedConditions: Floodplain along upper Slate River, anoxic subsurface conditions
   Equipment: Rhizon soil moisture samplers, aluminum fittings, phthalate tubing
2. Collect porewater samples
   Samples were extracted using valved syringes and immediately filtered through 0.22 μm, 0.1 μm (25 mm, PES, Sartorius) or 0.02 μm filter (25 mm, Anatop, Whatman) into N2-purged, glass serum vials having thick rubber stoppers to preserve redox conditions
   Quantities: Multiple samples collected at 0.22, 0.1, and 0.02 μm filtrationDuration: May-July 2021 sampling periodConditions: Samples shaded from sun exposure during extraction, subsurface anoxic conditions, then preserved at 4°C
   Equipment: valved syringes, 0.22 μm PES filters, 0.1 μm PES filters, 0.02 μm Anatop filters, N2-purged glass serum vials, thick rubber stoppers

Typical Equipment

• Rhizon soil moisture samplers
• valved syringes
• PES filters
• Anatop filters
• N2-purged glass serum vials

Output Measurements

• size-fractionated colloidal suspensions
• preserved redox conditions

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used