Anoxic TEM-EDS elemental mapping

Knowledge graph centered on Anoxic TEM-EDS elemental mapping with 21 nodes and 41 connections. Top connected: Bacteria, biogeochemical cycling, Structure and composition of natural ferrihydrite , iron speciation, uranium mineralogy.

Method synopsis

Transmission electron microscopy with energy dispersive X-ray spectroscopy performed under anoxic conditions using vacuum transfer holders to map elemental distributions in redox-sensitive particles.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Structure and composition of natural ferrihydrite nano-colloids in anoxic groundwater (2023), Water Research. Implementations in other papers (listed below) may differ.

1. Perform transmission electron microscopy
   Colloids from porewater solution (180 cm depth; 0.22 and 0.1 μm; sampled in May–July 2021) were deposited on a lacey carbon Cu grid (300 mesh) inside an anoxic chamber. Samples were transferred to the TEM under anoxic conditions using a Fischione vacuum transfer holder (Model 2560). The images were collected in both conventional bright field imaging mode using a CMOS Ceta camera and scanning mode with a high-angle annular dark-field (HAADF) detector
   Quantities: 300 mesh Cu gridDuration: Not specifiedConditions: Anoxic chamber conditions maintained throughout
   Equipment: lacey carbon Cu grids, Fischione vacuum transfer holder Model 2560, CMOS Ceta camera, HAADF detector

Typical Equipment

• lacey carbon Cu grids
• Fischione vacuum transfer holder Model 2560
• CMOS Ceta camera
• HAADF detector

Output Measurements

• elemental maps
• particle morphology
• compositional analysis

Papers Using This Protocol (1)

Species Studied With This Protocol