Pan-mammalian DNA methylation microarray profiling (Muridae)

Knowledge graph centered on Pan-mammalian DNA methylation microarray profiling (Muridae) with 31 nodes and 108 connections. Top connected: Pekania pennanti, Perymiscus species, small mammals, DNA methylation networks underlying mammalian trai, Peromyscus maniculatus.

Method synopsis

A microarray-based approach to profile DNA methylation at conserved CpG sites across diverse mammalian species without requiring species-specific reference genomes. Enables direct comparison of methylation patterns across taxonomically distant species.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — DNA methylation networks underlying mammalian traits (2023), Science. Implementations in other papers (listed below) may differ.

1. DNA methylation profiling using mammalian array
   Used a custom-designed mammalian methylation array platform that targets highly conserved cytosines (CpGs) in DNA sequences across mammalian species. Profiled 15,456 DNA samples derived from 70 tissue types from 348 mammalian species.
   Quantities: 15,456 samples from 348 species across 70 tissue typesDuration: Not specifiedConditions: Standard laboratory conditions for DNA methylation array processing
   Equipment: custom mammalian methylation array

Typical Equipment

• pan-mammalian DNA methylation microarray

Output Measurements

• DNA methylation levels
• CpG methylation patterns

Papers Using This Protocol (2)

Species Studied With This Protocol