16S rRNA gene sequencing (Nymphalidae)

Knowledge graph centered on 16S rRNA gene sequencing (Nymphalidae) with 45 nodes and 137 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee.

Method synopsis

Standard protocol to isolate, culture, and identify bacteria using genomic DNA extraction, 16S rRNA gene sequencing, and NCBI database comparison.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — The dispersal of microbes among and within flowers by butterflies (2023), Ecological Entomology. Implementations in other papers (listed below) may differ.

1. Isolate and identify test bacterium
   Isolated yellow bacterium from tarsi and proboscis of wild-caught S. mormonia by 3X serial transfers on Petri plates with HiMedia nutrient agar containing specific nutrients. Extracted genomic DNA using DNeasy Blood and Tissue Kit, performed 16S rRNA gene sequencing, and used NCBI Blast+ to identify as Rhodococcus fascians.
   Quantities: 3 serial transfers, whole genome sequencingDuration: Multiple transfers over unspecified timeConditions: Standard laboratory culture conditions
   Equipment: Petri plates, HiMedia nutrient agar, DNeasy Blood and Tissue Kit, Hi-Seq platform

Typical Equipment

• HiMedia nutrient agar
• DNeasy Blood and Tissue Kit
• Hi-Seq platform

Output Measurements

• bacterial species identification

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used