Synoptic watershed sampling for DOC export hotspot identification

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Method synopsis

Spatially distributed sampling campaign conducted during peak hydrologic conditions to identify locations of enhanced dissolved organic carbon export across a watershed transect.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure as described in the canonical source

Steps below were extracted from the paper that introduces this protocol — Disinfection byproducts formed during drinking water treatment reveal an export control point for dissolved organic matter in a subalpine headwater stream (2022), Water research X. Implementations in other papers (listed below) may differ.

1. Synoptic watershed sampling
   Synoptic sampling campaigns were conducted during peak flow events. Water samples were collected from approximately 20 locations along the Coal Creek transect including major tributaries. Samples were collected in pre-cleaned glass bottles and shipped on ice to Colorado School of Mines for processing within 48 hours.
   Quantities: Approximately 20 sampling locations across Coal Creek watershed transectDuration: Two days after peak flow events on June 8th, 2021 and July 28th, 2021, with additional sampling during falling limb on July 2020, 2021Conditions: Peak flow and falling limb conditions during snowmelt season
   Equipment: pre-cleaned glass bottles, ice
2. UV254 absorbance measurement
   All samples were analyzed for UV absorbance at 254 nm (UV254) with a subset receiving more comprehensive analyses. UV254 was calculated by normalizing values with the respective DOC concentrations in mg/L following Environmental Protection Agency Method 415.3.
   Quantities: All synoptic samples analyzed, subset for comprehensive analysisDuration: Within 48 hours of sample collectionConditions: Laboratory analysis at Colorado School of Mines
   Equipment: DU 800 Spectrophotometer

Typical Equipment

• pre-cleaned glass bottles
• DU 800 Spectrophotometer

Output Measurements

• spatial DOC concentrations
• UV254 values
• watershed export patterns

Papers Using This Protocol (2)

Species Studied With This Protocol

Places Where Used