Ovipositor bristle morphometry in Drosophilidae

Knowledge graph centered on Ovipositor bristle morphometry in Drosophilidae with 23 nodes and 82 connections. Top connected: Ligusticum porteri, Arabidopsis, Boechera stricta, Arabidopsis thaliana, Peromyscus maniculatus.

Method synopsis

Standardized counting and measurement of ventral ovipositor bristles using microscopy and digital imaging. Includes mounting specimens, photographing at standardized magnification, and counting specific bristle types.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Evolution and genomic basis of the plant penetrating ovipositor: a key morphological trait in herbivorous Drosophilidae (2022), Proceedings of the Royal Society B. Implementations in other papers (listed below) may differ.

1. Mount and photograph ovipositors
   Ovipositors were mounted on slides with Permount mounting medium and coverslips, and photographed using an EOS Rebel T3i camera mounted onto a Stemi 508 stereo microscope with a 1000 μm scale bar. Ovipositor length was measured using ImageJ.
   Quantities: 100 μm scale bar used for measurementsDuration: Not specifiedConditions: Standard microscopy conditions
   Equipment: Permount mounting medium, coverslips, EOS Rebel T3i camera, Stemi 508 stereo microscope, ImageJ software
2. Count ovipositor bristles
   Only ventral bristles (bristles 1-3 in 30), summed across both oviscapts were counted. Posterior bristles were excluded as they were largely invariable in number, and lateral bristles were excluded due to speculation that their involvement in leaf-cutting may be limited by their smaller size.
   Quantities: Bristles 1-3 counted across both oviscaptsDuration: Not specifiedConditions: Microscopic examination
   Equipment: microscope, imaging system

Typical Equipment

• Permount mounting medium
• EOS Rebel T3i camera
• Stemi 508 stereo microscope
• ImageJ software

Output Measurements

• ovipositor length
• ventral bristle count
• bristle morphology

Papers Using This Protocol (1)

Species Studied With This Protocol