full-sibling analysis (Pieridae)

Knowledge graph centered on full-sibling analysis (Pieridae) with 69 nodes and 176 connections. Top connected: Gothic, Pollinator-Plant Interactions, Pekania pennanti, Asteraceae, Lepidoptera.

Method synopsis

Multi-generation breeding program with controlled matings to estimate heritability of preference traits using pedigree-based models accounting for ZW sex determination system.

Synthesized from method descriptions across 3 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Novel host unmasks heritable variation in plant preference within an insect population (2022), Evolution. Implementations in other papers (listed below) may differ.

1. Laboratory maintenance and breeding
   Females were kept in 0.23 × 0.23 × 0.23 m screen cages and fed twice daily with 25% honey-water solution. Mating cages (30 × 45 × 45 cm net cages) were placed outdoors in direct sunlight with up to 20 individuals. Mating pairs were checked at least every 45 min and mating combinations recorded. Each male was mated with at least two females from different broods to produce half-sib families.
   Quantities: Up to 20 individuals per mating cage, over 1000 butterflies reared in lab, 138 F2 females in final datasetDuration: Multiple generations spanning each field seasonConditions: Room temperature in environmental chambers, 27-31°C during day and 20-22°C at night on 16:8 L:D cycle
   Equipment: 0.23 × 0.23 × 0.23 m screen cages, 30 × 45 × 45 cm net cages, environmental chambers
2. Larval rearing and sex determination
   Eggs briefly submerged in 0.075% hypochlorite solution, rinsed with water. After hatching, larvae developed on C. cordifolia which supports rapid development. Plants replaced as needed during larval development. After hardening of cuticle, sex determined and pupae grouped by sex and brood and left to emerge in screen cages.
   Quantities: All eggs from preference tests processed, over 1000 butterflies reared totalDuration: Full larval development period, emergence timing variedConditions: Environmental chamber conditions, C. cordifolia provided as food plant
   Equipment: 0.075% hypochlorite solution, C. cordifolia plants, screen cages for emergence

Typical Equipment

• mating cages
• environmental chambers
• screen cages

Output Measurements

• heritability estimates
• genetic variance components

Papers Using This Protocol (3)

Species Studied With This Protocol

Places Where Used