flow injection chemiluminescence

Knowledge graph centered on flow injection chemiluminescence with 16 nodes and 48 connections. Top connected: Bacteria, East River, Copper Creek, Phytoplankton, drive-point piezometer groundwater sampling.

Method synopsis

Flow injection chemiluminescence method for hydrogen peroxide quantification using FeLume system with acridinium ester reagent. Sensitive detection method for low-concentration H₂O₂ in natural waters.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Production of hydrogen peroxide in an intra-meander hyporheic zone at East River, Colorado (2022), Scientific Reports. Implementations in other papers (listed below) may differ.

1. H₂O₂ concentration determination
   H₂O₂ concentrations were determined by the chemiluminescence method using a flow injection FeLume system (Waterville Analytical, Waterville, ME). AE reagent of 5.0 µM at pH 3.0 was prepared each sampling day by dilution of a refrigerated AE stock solution (250 µM, pH 3.0) by adding 1.0 mM phosphate buffer (pH 3.0).
   Quantities: AE reagent: 5.0 µM at pH 3.0, stock solution: 250 µMDuration: Analysis within 4-6 min after collectionConditions: Laboratory analysis using refrigerated reagents
   Equipment: flow injection FeLume system, Waterville Analytical system

Typical Equipment

• flow injection FeLume system
• Waterville Analytical system

Output Measurements

• H₂O₂ concentrations

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used