microsatellite DNA analysis (Formicidae)

Knowledge graph centered on microsatellite DNA analysis (Formicidae) with 39 nodes and 125 connections. Top connected: Rocky Mountain Biological Laboratory, Engelmann Spruce, Salix lucida subsp. lasiandra, Selasphorus platycercus, Selasphorus rufus.

Method synopsis

DNA extraction from ant specimens followed by PCR amplification of microsatellite loci and relatedness estimation using maximum likelihood methods based on population allele frequencies.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• microcentrifuge tubes
• hand drill
• lysis buffer
• PCR reagents
• Techne Laboratories thermal cycler
• polyacrylamide gels

Output Measurements

• relatedness coefficients
• allele frequencies
• genetic diversity estimates

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used