Slingshot leaf sampling for genetic analysis (Salicaceae)

Knowledge graph centered on Slingshot leaf sampling for genetic analysis (Salicaceae) with 39 nodes and 125 connections. Top connected: Engelmann Spruce, Asteraceae, Artemisia tridentata, Populus tremuloides, Populus.

Method synopsis

Use of slingshots to collect fresh leaf material from tree canopies for downstream genetic analysis. Enables non-destructive sampling from tall trees.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Cytotype and genotype predict mortality and recruitment in Colorado quaking aspen (<i> Populus tremuloides </i>) (2021), Ecological Applications. Implementations in other papers (listed below) may differ.

1. Relocate and finalize plot positions
   Plots relocated from pre-chosen coordinates if found to be in small clearings. In such cases, chose nearest forest patch with at least 5m buffer to the clearing. Final plot coordinates measured using GPS units with nominal sub-meter resolution (in practice 1-2 meters due to topography and canopy cover). Each plot centered at a living, standing aspen stem minimum 5cm diameter at breast height, which was permanently tagged.
   Quantities: 503 plots, minimum 5cm DBH focal treesDuration: Summer 2018Conditions: Forest patches with canopy cover affecting GPS accuracy
   Equipment: Trimble Geo7X GPS units, permanent tree tags

Typical Equipment

• slingshots
• collection containers for leaf samples

Output Measurements

• leaf tissue samples

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used