BOSS microbial community preservation

Knowledge graph centered on BOSS microbial community preservation with 18 nodes and 61 connections. Top connected: Crested Butte, Pekania pennanti, East River, riparian vegetation, Archaea.

Method synopsis

Biological OsmoSampler System that preserves microbial communities in situ by adding preservative directly to the sample stream during collection. Enables molecular analysis of microbial communities from temporally inaccessible locations.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Hidden Processes During Seasonal Isolation of a High-Altitude Watershed (2021), Frontiers in Earth Science. Implementations in other papers (listed below) may differ.

1. Deploy BOSS for microbial sampling
   Biological OsmoSampler System (BOSS) used two OsmoSamplers connected in series, with one sampler collecting a sample and the second adding a preservative to the sample stream in situ to immediately preserve microbes present in the water for microbial community characterization. One OsmoSampler introduced 2x concentrated preservative (RNA/DNA Shield, Zymo Research, Irvine, CA, United States) into the sample stream at the inlet.
   Quantities: 2 OsmoSamplers connected in series, 0.25 mL day⁻¹ preservative addition rateDuration: September 2018 to December 2018Conditions: Late fall-early winter deployment and retrieval
   Equipment: BOSS system, RNA/DNA Shield preservative, Zymo Research preservative

Typical Equipment

• BOSS system
• RNA/DNA Shield preservative

Output Measurements

• preserved microbial DNA
• microbial community composition

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used