Factorial drought and nutrient stress experiment (Brassicaceae)

Knowledge graph centered on Factorial drought and nutrient stress experiment (Brassicaceae) with 72 nodes and 176 connections. Top connected: Hummingbird, Delphinium, Potentilla, Lepidoptera, Delphinium barbeyi.

Method synopsis

Greenhouse factorial experiment manipulating water availability (3 levels) and nutrient supply (2 levels) to test resource limitation effects on fitness components. Plants grown from field-collected seeds under controlled conditions with systematic treatment application.

Synthesized from method descriptions across 7 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Ecological factors influence balancing selection on leaf chemical profiles of a wildflower (2021), Nature Ecology & Evolution. The protocol was originally introduced by Resource availability alters fitness trade-offs: implications for evolution in stressful environments (2020), American Journal of Botany. Implementations in other papers (listed below) may differ.

1. Conduct greenhouse drought experiments
   Two controlled progressive dry-down experiments in the greenhouse tested for variation in drought response among CFR-NILs. Plants were grown for four months, then subjected to controlled drought by withholding water incrementally. Individual plants from genotypes across drought and well-watered treatments were measured for traits including height, leaf number, and water content.
   Quantities: 350 accessions from across species range in broad panel experiment, plus CFR-NIL genotypes in controlled crosses experimentDuration: 4 months growth plus progressive drought treatmentConditions: Controlled greenhouse environment with manipulated watering
   Equipment: greenhouse facilities, controlled watering system

Typical Equipment

• greenhouse benches
• misting system
• hand watering equipment
• Peters Professional General Purpose Fertilizer 20N-10P-20K
• controlled temperature chamber

Output Measurements

• survival
• probability of reproduction
• fecundity
• relative growth rate

Papers Using This Protocol (7)

Species Studied With This Protocol