Factorial drought and nutrient stress experiment (Brassicaceae)

Knowledge graph centered on Factorial drought and nutrient stress experiment (Brassicaceae) with 72 nodes and 169 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Barbey's Larkspur, Nuttall's Larkspur, Larkspurs.

Method synopsis

Greenhouse factorial experiment manipulating water availability (3 levels) and nutrient supply (2 levels) to test resource limitation effects on fitness components. Plants grown from field-collected seeds under controlled conditions with systematic treatment application.

Synthesized from method descriptions across 7 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Ecological factors influence balancing selection on leaf chemical profiles of a wildflower (2021), Nature Ecology & Evolution. The protocol was originally introduced by Resource availability alters fitness trade-offs: implications for evolution in stressful environments (2020), American Journal of Botany. Implementations in other papers (listed below) may differ.

1. Conduct greenhouse drought experiments
   Two controlled progressive dry-down experiments in the greenhouse tested for variation in drought response among CFR-NILs. Plants were grown for four months, then subjected to controlled drought by withholding water incrementally. Individual plants from genotypes across drought and well-watered treatments were measured for traits including height, leaf number, and water content.
   Quantities: 350 accessions from across species range in broad panel experiment, plus CFR-NIL genotypes in controlled crosses experimentDuration: 4 months growth plus progressive drought treatmentConditions: Controlled greenhouse environment with manipulated watering
   Equipment: greenhouse facilities, controlled watering system

Typical Equipment

• greenhouse benches
• misting system
• hand watering equipment
• Peters Professional General Purpose Fertilizer 20N-10P-20K
• controlled temperature chamber

Output Measurements

• survival
• probability of reproduction
• fecundity
• relative growth rate

Papers Using This Protocol (7)

Species Studied With This Protocol

Places Where Used