Factorial phenology-water manipulation of potted wildflowers

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Method synopsis

A factorial experiment manipulating both flowering phenology (by moving plants between elevations) and water availability (through differential watering regimes) using potted native plants in field arrays. Plants were held at high elevation to delay flowering, then moved to lower elevation in weekly cohorts to create staggered flowering times.

Synthesized from method descriptions across 5 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Experimental Test of the Combined Effects of Water Availability and Flowering Time on Pollinator Visitation and Seed Set (2021), Frontiers of Ecology & Evolution. The protocol was originally introduced by The Effects of Climate-Driven Changes in Co-flowering between <i>Linum lewisii</i> and <i>Potentilla pulcherrima</i> on Pollinator Services (2018). Implementations in other papers (listed below) may differ.

1. Collect and pot experimental plants
   Between 2012 and 2015, 120 plants were collected from a large M. ciliata population at Rustler Gulch and potted using native soil in 2-gallon plastic pots (actual volume: 1.593 Gallons, Nursery Supplies, Inc.). A portion of the plants were used for other studies in 2013 and 2014; the others remained in trenches and overwintered in the ground under snow at RMBL until their use for this experiment in 2016.
   Quantities: 120 plants total, 2-gallon plastic pots (1.593 gallon actual volume)Duration: Collection occurred between 2012 and 2015Conditions: Plants collected from Rustler Gulch natural population
   Equipment: 2-gallon plastic pots, native soil
2. Establish phenology manipulation
   In 2016, potted plants were randomly assigned to one of four flower treatments: dry, average, and wet. To inhibit flowering, potted plants were moved to Schofield Pass (39°00'54.98" N, 107° 2'49.40" W; 3,263 m a.s.l.) in early June where they were kept in a shaded snowbank under a mesh shade-shelter.
   Quantities: 4 phenology treatment weeks, plants moved to 3,263 m elevationDuration: Moved to Schofield Pass in early June, held there until assigned flowering weekConditions: Shaded snowbank conditions at high elevation to prevent flowering

Typical Equipment

• 2-gallon plastic pots
• mesh shade-shelter
• Campbell Scientific HydroSense probe
• watering cans

Output Measurements

• flowering phenology
• soil moisture
• reproductive success

Papers Using This Protocol (5)

Species Studied With This Protocol

Places Where Used