neutrophil:lymphocyte ratio (Sciuridae)

Knowledge graph centered on neutrophil:lymphocyte ratio (Sciuridae) with 71 nodes and 162 connections. Top connected: Marmota flaviventris, mark-recapture (Sciuridae), focal animal sampling (Sciuridae), antipredator behavior, Zonotrichia leucophrys.

Method synopsis

Blood collection and analysis to determine neutrophil and lymphocyte counts for calculating neutrophil-to-lymphocyte ratios as a measure of immune response activity.

Synthesized from method descriptions across 7 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — How do humans impact yellow-bellied marmots? An integrative analysis (2021), Applied Animal Behaviour Science. Implementations in other papers (listed below) may differ.

1. Measure neutrophil-lymphocyte ratios
   A drop of blood was used to make a thin layer blood smear, dyed it with a Hema-3 Stat kit and counted the number of neutrophils, lymphocytes, monocytes, and basophils until 100 cells were scored or 30 min elapsed. The number of neutrophils were then divided by number of lymphocytes for analysis.
   Quantities: 100 cells counted or 30 minute time limit per sampleDuration: 30 minute maximum counting time per sampleConditions: Blood smears prepared immediately from fresh blood samples
   Equipment: Hema-3 Stat kit, microscope for cell counting

Typical Equipment

• Tomahawk traps
• isoflurane gas
• blood collection equipment
• blood analysis equipment

Output Measurements

• neutrophil-to-lymphocyte ratio

Papers Using This Protocol (7)

Species Studied With This Protocol