Kellogg Biological Station pH protocol

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Method synopsis

Standard suite of sediment physical and chemical analyses including bulk density, pH, loss-on-ignition, and root biomass content to characterize biogeochemical properties of pond sediments.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Biogeochemical characteristics and hydroperiod affect carbon dioxide flux rates from exposed high-elevation pond sediments (2020), Limnology and Oceanography. Implementations in other papers (listed below) may differ.

1. Collect sediment cores for laboratory analysis
   Collect sediment cores (5.6 cm diameter, 7 cm depth) from within the collars and transport them to the lab on ice, where they were refrigerated at 4°C for 12-24 h until ensuing analyses could be completed. Weigh entire sample, homogenize the moist sample by mixing, then allocate subsamples to different analyses.
   Quantities: 5.6 cm diameter, 7 cm depth cores. Collected on July 11 and July 17 (montane) and September (subalpine)Duration: Single collection event per siteConditions: Transported on ice, refrigerated at 4°C for 12-24 hours
   Equipment: sediment corer, ice, refrigeration
2. Measure sediment bulk density and moisture
   Allocate 5 g of the sample to measuring gravimetric sediment moisture by drying the sample at 60°C for 48 h and measuring mass lost by drying. Calculate bulk density (BD) by multiplying the entire field moist sample mass by the dry-to-field moist ratio of the sediment and dividing by the volume of the sediment core (172.41 cm³).
   Quantities: 5 g subsample, 48 hours drying at 60°C, 172.41 cm³ core volumeDuration: 48 hours drying timeConditions: 60°C drying temperature
   Equipment: drying oven, analytical balance
3. Measure sediment pH
   Measure sediment pH using the protocol of the Kellogg Biological Station that required two 15 g field-moist subsamples to be mixed in 30 mL of deionized water (DI) each for 30 minutes.

Typical Equipment

• sediment corer
• drying oven
• AccumetAB62 pH meter (Fisher Scientific)
• muffle furnace
• 1-mm sieve mesh

Output Measurements

• bulk density
• sediment pH
• loss-on-ignition
• root biomass

Papers Using This Protocol (1)

Species Studied With This Protocol