Transect-based seed sampling for plant population genetics (Ranunculaceae)

Knowledge graph centered on Transect-based seed sampling for plant population genetics (Ranunculaceae) with 34 nodes and 135 connections. Top connected: Rocky Mountain Biological Laboratory, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee, Buff-tailed Bumble Bee.

Method synopsis

Systematic collection of seeds from plants along established transects for genetic analysis. Seeds collected within defined distances of sampling points and pooled by maternal plant.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Foraging efficiency and size matching in a plant-pollinator communitiy: the importance of sugar content and tongue length (2019), Ecology letters. Implementations in other papers (listed below) may differ.

1. Establish transect layout
   Two transects were laid out at right angles in a representative meadow, each 160m long, forming a cross pattern
   Quantities: 2 transects, 160m length each, laid at right anglesDuration: Not specifiedConditions: June 1981, at 3000m elevation meadow
   Equipment: measuring equipment for transect layout
2. Establish sampling points
   Sampling points were established every 5m along both transects, with the longest intersample distance being 213m
   Quantities: 26 sampling points total, spaced every 5m, maximum distance 213m between pointsDuration: Not specifiedConditions: June 1981
   Equipment: measuring tape or similar for 5m spacing
3. Collect seed samples
   Mature seeds were harvested from plants growing within 1m of each sampling point. Seeds from different fruits on a plant were pooled, and only 3-6 plants could be sampled per transect point due to herbivory and fruit abortion
   Quantities: Seeds from 3-6 plants per sampling point, within 1m radius, total area sampled about 3m², all 26 samples involved seeds from >2 plants, half involved seeds from >4 plants Not specified

Typical Equipment

• measuring equipment for transect layout
• measuring tape
• collection containers

Output Measurements

• seed samples by maternal plant
• spatial coordinates of samples

Papers Using This Protocol (2)

Species Studied With This Protocol

Places Where Used