Comparative soil respiration model evaluation (Plantae)

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Method synopsis

Systematic comparison of first-order kinetic vs. dormancy-based models for predicting soil CO2 respiration under varying moisture conditions using multi-scale experimental datasets.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Modeling transient soil moisture limitations on microbial carbon respiration (2019), Journal of Geophysical Research: Biogeosciences. Implementations in other papers (listed below) may differ.

1. Soil sample collection - first batch
   Three samples were taken at coarse resolution of ~50-cm intervals from a hand-augured hole to a maximum depth of 165 cm. The shallow sample (0–52 cm) is primarily a mixture of the A1 and A2 horizon, the intermediate sample (63–108 cm) represents a mixture of horizons A/B, B/C, and Cw1, and the deep sample (112–165 cm) contains soil from the Cw2 horizon.
   Quantities: 3 samples representing depth intervals 0-52 cm, 63-108 cm, and 112-165 cmDuration: Single sampling eventConditions: November 2015, subalpine meadow along Bradley Creek
   Equipment: hand auger
2. Soil sample collection - second batch
   Five samples were collected from a soil pit located within 3 m of the original sampling location with 10-cm depth interval, to a maximum depth of 50 cm. These samples represent the mixture of OA and A1 horizon (0–10 cm), A1 (10–20 and 20–30 cm), the mixture of A1 and A2 (30–40 cm), and A2 (40–50 cm).
   Quantities: 5 samples at 10-cm intervals: 0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm, 40-50 cmDuration: Single sampling eventConditions: June 2016, within 3 m of original sampling location
   Equipment: soil pit excavation tools
3. Sample preparation
   Samples were sealed in plastic bags and kept under cool, dark conditions until processing could be completed. Prior to incubations, all soils were air dried for 2 weeks at ambient temperature (22°C) before they were crushed and sieved to 2 mm to remove the coarse fraction, consisting of large stones and biological material.

Typical Equipment

• computational modeling software
• parameter optimization software
• statistical software

Output Measurements

• CO2 respiration rates
• model performance metrics
• AIC values

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used