Knowledge graph centered on Refractometric nectar analysis with 72 nodes and 247 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee.
Method synopsis
A field-based protocol for extracting nectar from flowers that have been exposed to bee visitation, then quantifying ethanol content using enzyme-based assays. Flowers are pre-isolated to prevent nectar depletion before sampling.
Synthesized from method descriptions across 25 papers using this protocol.
Procedure from a recent peer-reviewed implementation
Used microcapillary tubes to measure nectar volume per flower. Measured nectar standing crop on same day of observation and at same time of day as bee observations. Calculated total nectar volume per flower (median range 4-21 plants per observation period) and flowers measured per observation (range 19-212 flowers).
Quantities: 4-21 plants per observation period, 19-212 flowers measured per observation period, median 7 flowers per plantDuration: Measured on same day and time as bee observationsConditions: Same environmental conditions as bee observation periods