Hybrid zone cline analysis over elevational gradient (Polemoniaceae)

Knowledge graph centered on Hybrid zone cline analysis over elevational gradient (Polemoniaceae) with 36 nodes and 128 connections. Top connected: Hummingbird, Bombus, Ipomopsis, I. aggregata, Ipomopsis aggregata.

Method synopsis

Long-term monitoring of plant morphological traits across permanent transects spanning a hybrid zone between two Ipomopsis species along an elevational gradient, with cline fitting to quantify trait gradients.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Clines in traits compared over two decades in a plant hybrid zone (2018), Annals of Botany. Implementations in other papers (listed below) may differ.

1. Establish permanent transects and mark individual plants
   Set up 30-m transects across 12 populations along the hybrid zone. Mark individual plants at every 3-m interval along transects with permanent flags. In populations that no longer spanned 30 m in 2015, establish second 30-m transect. Include a second 30-m transect at the lowest elevation (pure I. aggregata) population.
   Quantities: 12 populations total, 30-m transects, plants marked every 3 m (every 1-m in 2015-2016), 500 total plants measured across all yearsDuration: Initial setup in 1991, maintained through 2016Conditions: Elevational gradient spanning hybrid zone from lowest-elevation I. aggregata populations to highest-elevation I. tenuittuba populations
   Equipment: permanent flags, GPS
2. Measure morphological traits on marked plants
   Measure corolla length, corolla width, and maximum stamen length on nearest flowering individual to each mark. Average measurements over three or more flowers per plant. Determine anther insertion by subtracting maximum stamen length from corolla length. Assess petal color, nectar production rate, specific leaf area (SLA), and leaf trichome density.
   Quantities: Measurements on marked plants at 3-m intervals (1-m intervals in 2015-2016), averaged over 3+ flowers per plantDuration: Annual measurements from 1991-1992, then 2015-2016Conditions: During flowering period when plants have multiple open flowers
   Equipment: measuring tools for corolla and stamen length, reflectance spectrometer, flatbed scanner, ImageJ freeware

Typical Equipment

• permanent flags
• GPS
• measuring tools
• reflectance spectrometer
• flatbed scanner
• ImageJ freeware
• SAS statistical software version 9.3

Output Measurements

• corolla length
• corolla width
• anther insertion
• petal color
• nectar production rate
• specific leaf area
• leaf trichome density

Papers Using This Protocol (2)

Species Studied With This Protocol