Ion exchange resin strips

Knowledge graph centered on Ion exchange resin strips with 39 nodes and 124 connections. Top connected: Delphinium, Potentilla, Erigeron, elevational gradients, Helianthella.

Method synopsis

Mixed-bed ion-exchange resin bags deployed at 5 cm soil depth to assess NO3-N and NH4-N availability. Resins are extracted with KCl and analyzed using flow injection autoanalyzer.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Plant dominance in a subalpine montane meadow: biotic vs. abiotic controls of subordinate diversity within and across sites (2018), PeerJ. Implementations in other papers (listed below) may differ.

1. Measure soil nitrogen availability
   To assess the availability of NO3-N and NH4-N in the soil solution, mixed-bed ion-exchange resin bags were placed in nylon stockings (H-OH form, # R231-500; Fisher Scientific International Inc., Pittsburgh, PA, USA) at five-cm soil depth at two locations in each of the experimental and observational plots. Resins were deployed in early June and retrieved from plots in late July. Resins were then air-dried, and two g of resins from each plot were extracted with 2 M KCl. Pool sizes of NO3- and NH4+ were analyzed on a Lachat AE Flow Injection Autoanalyzer (Lachat Quikchem 8000; Hach Corporation, Loveland, OH).
   Quantities: 2 locations per plot, 5 cm soil depth, 2 g resins extracted with 2 M KClDuration: Deployed early June, retrieved late JulyConditions: Buried at 5 cm soil depth
   Equipment: mixed-bed ion-exchange resin bags, nylon stockings (H-OH form, # R231-500; Fisher Scientific International Inc., Pittsburgh, PA, USA), Lachat AE Flow Injection Autoanalyzer (Lachat Quikchem 8000; Hach Corporation, Loveland, OH)

Typical Equipment

• mixed-bed ion-exchange resin bags
• nylon stockings (H-OH form, # R231-500; Fisher Scientific International Inc., Pittsburgh, PA, USA)
• Lachat AE Flow Injection Autoanalyzer (Lachat Quikchem 8000; Hach Corporation, Loveland, OH)

Output Measurements

• soil NO3-N availability
• soil NH4-N availability

Papers Using This Protocol (2)

Species Studied With This Protocol