tetrazolium test (Brassicaceae)

Knowledge graph centered on tetrazolium test (Brassicaceae) with 24 nodes and 95 connections. Top connected: elevational gradients, Arabidopsis, Boechera stricta, Boechera, climate change effects on species interactions.

Method synopsis

Standard biochemical assay using 2,3,5-triphenyltetrazolium chloride staining to distinguish between dormant and nonviable seeds that failed to germinate.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Transgenerational and within-generation plasticity in response to climate change: insights from a manipulative field experiment across an elevational gradient (2018), Am. Nat.. Implementations in other papers (listed below) may differ.

1. Seed viability assessment
   Evaluated viability of seeds that did not germinate by extracting embryos from seed coats and soaking in 1% solution of 2,3,5-triphenyltetrazolium chloride. After 48 hours, assigned dormant status to seeds if radicle and at least one cotyledon had stained pink, considered embryos that did not stain as nonviable.
   Quantities: N = 2,486 seeds assessed for viability, 156 seeds excluded due to contamination by moldDuration: 48 hour staining periodConditions: Laboratory conditions for tetrazolium test
   Equipment: 2,3,5-triphenyltetrazolium chloride solution

Typical Equipment

• 2,3,5-triphenyltetrazolium chloride solution

Output Measurements

• seed viability status
• dormancy classification

Papers Using This Protocol (1)

Species Studied With This Protocol