Carbon isotope discrimination analysis

Knowledge graph centered on Carbon isotope discrimination analysis with 74 nodes and 153 connections. Top connected: Hummingbird, Ipomopsis, Delphinium, I. aggregata, Ipomopsis aggregata.

Method synopsis

Measurement of water-use efficiency differences between plant sexes using leaf carbon isotope ratios (δ13C) to understand mechanistic basis of sex-specific climate responses.

Synthesized from method descriptions across 10 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Can flowers affect land surface albedo and soil microclimates? (2021), International Journal of Biometeorology. Implementations in other papers (listed below) may differ.

1. Collect leaf samples for water stress analysis
   One leaf each from two non-flowering individuals and one leaf each from two flowering individuals per plot (four leaves per plot) were collected for analysis of carbon isotope ratios (Δ13C). Leaves were collected after flowering finished, still green and healthy-looking, avoiding signs of herbivory or disease.
   Quantities: 4 leaves per plot, 2 from flowering and 2 from non-flowering plantsDuration: Single collection after flowering in 2015 and 2017Conditions: After flowering finished, leaves still green and healthy
   Equipment: small paper envelopes, MP FastPrep-24 homogenizer, drying equipment
2. Measure leaf water pressure
   Four leaves were sampled from four different individuals per plot (two flowering, two non-flowering plants) during peak flowering in 2017. Leaves were cut at base of petiole and immediately inserted into pressure chamber sample chamber of PMS instrument.
   Quantities: 4 leaves per plot from 4 different individuals, 2 flowering and 2 non-flowering plantsDuration: Single measurement campaign during peak flowering 2017 (July 19-27)Conditions: During peak flowering period
   Equipment: PMS model 1505D pressure chamber

Typical Equipment

• water potential measurement device
• stomatal conductance measurement device
• soil moisture sensor

Output Measurements

• water potential
• stomatal conductance
• soil moisture

Papers Using This Protocol (10)

Species Studied With This Protocol