Butterfly egg biochemical composition analysis (Nymphalidae)

Knowledge graph centered on Butterfly egg biochemical composition analysis (Nymphalidae) with 33 nodes and 113 connections. Top connected: Bombus, Ligusticum porteri, Bombus terrestris, Bombus appositus, Bombus impatiens.

Method synopsis

Multi-component analysis of butterfly egg composition including protein content via Bradford dye-binding method, glycogen content via modified Giron protocol, and triglyceride content using commercial reagent kits.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Effects of increased flight on the energetics and life history of the butterfly <i>Speyeria mormonia</i> (2015), PLoS One. Implementations in other papers (listed below) may differ.

1. Analyze egg chemistry
   Quantified lipid, glycogen and protein content, and dry mass of eggs collected at ages 4, 5, 7, 11, 13, 17, and 19 days. Egg protein content quantified for one egg from each age using Bradford dye-binding method following modified protocols. Quantified glycogen content of pooled sample of 4 eggs from each age following modified protocol. For egg triglycerides used pooled sample of 3 to 4 eggs from each age using standard protocol for Infinity Triglycerides Stable Reagent.
   Quantities: Eggs analyzed at 7 different time points, 1 egg for protein, 4 eggs pooled for glycogen, 3-4 eggs pooled for triglyceridesDuration: At specific ages: 4, 5, 7, 11, 13, 17, and 19 daysConditions: Laboratory analysis conditions
   Equipment: Bradford dye-binding reagents, Infinity Triglycerides Stable Reagent (ThermoScientific, Waltham, MA, USA)

Typical Equipment

• Bradford dye-binding reagents
• Infinity Triglycerides Stable Reagent (ThermoScientific, Waltham, MA, USA)
• drying oven

Output Measurements

• egg protein content
• egg glycogen content
• egg triglyceride content
• egg dry mass

Papers Using This Protocol (1)

Species Studied With This Protocol