Frequency of dividing cells analysis (Gomphonemataceae)

Knowledge graph centered on Frequency of dividing cells analysis (Gomphonemataceae) with 18 nodes and 65 connections. Top connected: Atriplex canescens, aquatic insects, native species, fish and wildlife, nutrient limitation.

Method synopsis

Microscopic analysis of D. geminata samples to quantify the percentage of cells undergoing division as an indicator of phosphorus-limited growth rates.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — The Didymo story: the role of low dissolved phosphorus in the formation of <i>Didymosphenia geminata</i> blooms (2014), Diatom Research. Implementations in other papers (listed below) may differ.

1. Measure frequency of dividing cells
   Used frequency of dividing cells (FDC) as a metric for phosphorus-limited growth rates in D. geminata populations. FDC measurements were taken from field samples to assess cellular division rates under different phosphorus concentrations.
   Quantities: FDC measurements from multiple river sites with D. geminata presentDuration: During field sampling periodsConditions: Various ambient phosphorus concentration conditions
   Equipment: microscopy equipment for cell division analysis

Typical Equipment

• microscopy equipment for cell division analysis

Output Measurements

• frequency of dividing cells percentage

Papers Using This Protocol (1)

Species Studied With This Protocol