standard microbiology protocols

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Method synopsis

Laboratory protocol for preparing yeast-inoculated synthetic nectar with species-specific concentrations and incubation conditions. Synthetic nectar designed to match natural nectar composition.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Nectar Yeasts in the Tall Larkspur <i>Delphinium barbeyi</i> (Ranunculaceae) and Effects on Components of Pollinator Foraging Behavior (2014), PloS one. Implementations in other papers (listed below) may differ.

1. Count yeast cells using microscopy
   Cell density (cells/mm³ of nectar) was determined using a Neubauer chamber (Hausser Scientific, Horsham, PA, USA) and standard cell counting methods at a magnification of 400x (Nikon Eclipse E400, Melville, NY, USA). Identified cells as yeasts based on size and morphological features, such as the presence of budding cells and large vacuoles, which contain refractive corpuscles.
   Quantities: Cell density measured as cells/mm³ of nectar at 400x magnificationDuration: Standard microscopy processing timeConditions: Laboratory conditions using standard cell counting chamber
   Equipment: Neubauer chamber, Hausser Scientific chamber, Nikon Eclipse E400 microscope

Typical Equipment

• synthetic nectar
• yeast cultures
• 25 μl microcapillary tubes
• 50 μl Hamilton syringe

Output Measurements

• yeast cell concentration
• nectar composition

Papers Using This Protocol (4)

Species Studied With This Protocol

Places Where Used