Knowledge graph centered on D1/D2 rRNA gene sequencing (Ranunculaceae) with 38 nodes and 123 connections. Top connected: Hummingbird, Bombus, Delphinium, Bombus terrestris, Bombus appositus.
Yeast isolates were cultured on chloramphenicol-supplemented YM agar and identified through PCR amplification and sequencing of the D1/D2 domains of the large subunit nuclear ribosomal RNA gene using primers NL1 and NL4.
Synthesized from method descriptions across 1 paper using this protocol.
Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Nectar Yeasts in the Tall Larkspur <i>Delphinium barbeyi</i> (Ranunculaceae) and Effects on Components of Pollinator Foraging Behavior (2014), PloS one. Implementations in other papers (listed below) may differ.