Knowledge graph centered on anogenital distance measurement (Sciuridae) with 30 nodes and 114 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Marmota, Yellow-bellied Marmot, Colorado East River Valley.
Method synopsis
Measurement of anogenital distance using dial or digital callipers to 1mm accuracy as a proxy for prenatal testosterone exposure and masculinization. Distance measured from centre of genital opening to centre of anus.
Synthesized from method descriptions across 2 papers using this protocol.
Procedure from a recent peer-reviewed implementation
Marmots were individually trapped and marked with unique symbols on their back using hair dye to facilitate behavioral observations. Each individual was weighed and the anogenital (AG) distance was measured (distance from center of genital papilla to anus).
Quantities: 202 female marmots total: 110 juveniles and 92 yearlings from 89 different littersDuration: Conducted regularly from 2002 to 2009Conditions: During active season from natal emergence to hibernation
Equipment: traps, hair dye, measuring tools
AG distance index calculation
AG distance measurements were standardized by extracting residuals from a linear regression of AG distance against body mass to create an AG distance index independent of body size effects.
Quantities: Calculated for all 202 individualsDuration: Post-processing analysisConditions: Statistical analysis phase