Alpine lake water chemistry sampling

Knowledge graph centered on Alpine lake water chemistry sampling with 15 nodes and 34 connections. Top connected: Nutrient availability and phytoplankton nutrient l, Daphnia, nitrogen pollution, nutrient limitation, algae bloom.

Method synopsis

Standardized collection of lake water samples from deepest point using submersible pumps with macrozooplankton exclusion. Includes physical profiling and immediate chemical processing.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Nutrient availability and phytoplankton nutrient limitation across a gradient of atmospheric nitrogen deposition (2009), Ecology. Implementations in other papers (listed below) may differ.

1. Water sample collection
   Water samples collected from deepest part of lake using portable echo sounder to locate maximum depth, then sampling from ~1-1.5 m depth using battery-powered submersible pump fitted with tubing. Samples pumped through 80-μm mesh Nitex filters to remove ambient macrozooplankton.
   Quantities: 4 independent replicate samples per lakeDuration: Single sampling event per lake during summer 2006Conditions: Morning sampling to avoid afternoon thunderstorms. High-deposition lakes sampled in early July and August, low-deposition lakes in late July and late August/early September
   Equipment: battery-powered submersible pump, tubing, 80-μm mesh Nitex filters, portable echo sounder, lakewater-rinsed cubitainers
2. Physical and chemical analysis
   Temperature and dissolved oxygen profiles measured at 1-m intervals using YSI model 85 temperature-oxygen probe. pH determined using Accumet AR10 pH meter. Water samples analyzed for ammonia/ammonium, nitrate + nitrite, soluble reactive phosphorus using standard methods including indophenol colorimetric and segmented flow analysis.
   Quantities: Measurements at 1-m depth intervals, 4 replicate water samples per lakeDuration: Within 2-4 hours of sampling for processingConditions: Samples returned immediately to laboratory and processed within hours
   Equipment: YSI model 85 temperature-oxygen probe, Accumet AR10 pH meter, OI Analytical Flow Solution IV Instrument, Metrohm 761 Compact Ion Chromatography instrument, Metrohm A Supp 5 anion column

Typical Equipment

• battery-powered submersible pump
• YSI model 85 temperature-oxygen probe
• Accumet AR10 pH meter
• 80-μm mesh Nitex filters

Output Measurements

• temperature profiles
• dissolved oxygen profiles
• pH
• nutrient concentrations
• total phosphorus
• total nitrogen

Papers Using This Protocol (1)

Species Studied With This Protocol