micro-Dumas combustion analysis (Membracidae)

Knowledge graph centered on micro-Dumas combustion analysis (Membracidae) with 23 nodes and 93 connections. Top connected: Ligusticum porteri, Asteraceae, Lepidoptera, Artemisia, space use.

Method synopsis

Laboratory analysis of total carbon and nitrogen content in small arthropod specimens using dry micro-Dumas combustion followed by GC-MS analysis with acetamilde and atropine standards for normalization.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Insect herbivore stoichiometry: the relative importance of host plants and ant mutualists (2008), Ecological Entomology. Implementations in other papers (listed below) may differ.

1. Analyze carbon and nitrogen content
   Sent samples to Stable Isotope/Soil Biology Laboratory at University of Georgia for total carbon and nitrogen content analysis by dry micro-Dumas combustion analysis using GC-MS with 15-point normalization using acetamilde and atropine
   Quantities: 122 individual nymph samples analyzedDuration: Laboratory analysis timeline not specifiedConditions: Standard laboratory analytical conditions
   Equipment: GC-MS, micro-Dumas combustion analyzer

Typical Equipment

• GC-MS
• micro-Dumas combustion analyzer

Output Measurements

• percent carbon
• percent nitrogen

Papers Using This Protocol (1)

Species Studied With This Protocol