NRCS soil characterization methods (Plantae)

Knowledge graph centered on NRCS soil characterization methods (Plantae) with 59 nodes and 170 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Barbey's Larkspur, Nuttall's Larkspur, Larkspurs.

Method synopsis

Random plot establishment along elevational gradients followed by soil coring and root extraction to quantify coarse and fine root biomass allocation patterns. Ground cover composition is estimated to correlate with root allocation patterns.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Experimental warming decouples plant-fungal symbiont interactions and leads to a more conservative ecosystem (2026), PNAS. The protocol was originally introduced by An investigation into the effects of arbuscular mycorrhizal fungi (AMF) to dark septate endophytes (DSE) ratio on the coarse root to fine root ratio at varying elevation in the rocky mountains (2020). Implementations in other papers (listed below) may differ.

1. Collect rhizosphere soil and root samples
   Researchers collected plant-specific rhizosphere soil cores (2.5 × 5 cm) and measured aboveground stem and leaf biomass, root traits for three randomly selected individuals of each focal species within each plot. They collected root and bulk soil samples in 10 cm increments to a 1-m depth (10 total samples across depth for each plot).
   Quantities: 3 individuals per focal species per plot, soil cores 2.5 × 5 cm, 10 cm depth increments to 1 m depth, 10 total samples per plotDuration: Single sampling event after 29 yearsConditions: During growing season
   Equipment: soil coring tools

Typical Equipment

• soil core
• 2mm pore sieve
• forceps
• calipers
• DI water
• quadrant frame

Output Measurements

• coarse root biomass
• fine root biomass
• fine to coarse root ratio
• ground cover by species

Papers Using This Protocol (4)

Species Studied With This Protocol

Places Where Used