Drosophila courtship song recording via wing vibration (Drosophilidae)

Knowledge graph centered on Drosophila courtship song recording via wing vibration (Drosophilidae) with 31 nodes and 84 connections. Top connected: frequency-dependent selection, The extent of variation in male song, wing and gen, Drosophila melanogaster, Poecilia reticulata, ecological speciation.

Method synopsis

Recording male Drosophila courtship songs by placing flies in Petri dish chambers and capturing wing vibration sounds with directional microphone positioned above the chamber roof.

Synthesized from method descriptions across 3 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Male courtship song and female preference variation between phylogeographically distinct populations of <i>Drosophila montana</i> (2007), Evolution. Implementations in other papers (listed below) may differ.

1. Record male courtship song
   Males were stimulated to produce courtship song by placing them with a mature virgin female of the same strain into a small net covered petri dish. Song was recorded using an AKG C 1000 S microphone and a Marantz Professional tape recorder (model no. 74PDM 502/02B). Males from different populations and strains were recorded in a random order at a constant ambient temperature of 20°C.
   Quantities: Males from nine lines from the Oulanka population, 23 lines from the Vancouver, and six lines from the Colorado. Song traits were analyzed using the Signal 4.0 sound analysis systemDuration: Recording sessions conducted at constant temperatureConditions: Constant ambient temperature of 20°C
   Equipment: AKG C 1000 S microphone, Marantz Professional tape recorder model no. 74PDM 502/02B, small net covered petri dish, Signal 4.0 sound analysis system
2. Analyze song carrier frequency
   The carrier frequency was taken as the highest peak frequency from a fast Fourier transformation (FFT) transform within each of three separate pulse trains whenever available. The songs of approximately three F1 males per line were recorded and the average of three pulse trains for each male was used as the unit of analysis in a fully nested analysis of variance (ANOVA).
   Quantities: Approximately three F1 males per line, average of three pulse trains per male, 115 males total (Z = 0.894, P = 0.4)Duration: Analysis conducted on recorded songsConditions: Frequency data were normally distributed and thus used untransformed

Typical Equipment

• Petri dish
• nylon net
• AKG C 1000 S microphone
• Marantz Professional tape recorder
• Signal 4.0 sound analysis system

Output Measurements

• pulse number
• pulse train length
• sound pulse length
• interpulse interval
• cycles in sound pulse
• carrier frequency

Papers Using This Protocol (3)

Species Studied With This Protocol