Reproductive effort carbon budget model (Asteraceae)

Knowledge graph centered on Reproductive effort carbon budget model (Asteraceae) with 44 nodes and 135 connections. Top connected: Delphinium, Spruce, phenological mismatch, Asteraceae, Potentilla.

Method synopsis

Integrative model combining photosynthesis measurements, respiration estimates, and total nonstructural carbohydrate analysis to calculate carbon allocation to reproduction.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Reproductive and physiological responses to simulated climate warming for four subalpine species (2007), New Phytologist. Implementations in other papers (listed below) may differ.

1. Collect plant material for TNC analysis
   We measured TNC values of plant tissues to estimate the amount of nonstructural carbohydrates translocated from vegetative to reproductive tissues. To prevent damage to plants in the experimental plots, TNC values were measured on plants collected from outside the experimental plots. We assumed that the TNC values for these plants were representative of those in both the control and heated plots. Five plants were collected for each species during each of the developmental stages, coinciding with measurements of leaf gas exchange in the plots. Root, leaf, and floral/fruit tissues were separated, dried, ground to a fine powder with a ball mill grinder, and analyzed for TNC following Tissue et al. (1995).
   Quantities: 5 plants per species per developmental stageDuration: Collections coinciding with gas exchange measurementsConditions: Plants collected from outside experimental plots
   Equipment: ball mill grinder

Typical Equipment

• ball mill grinder

Output Measurements

• reproductive effort
• carbon allocation

Papers Using This Protocol (1)

Species Studied With This Protocol